Insights Into Transgenerational Effect of Deltamethrin on Callosobruchus Chinensis (L.)
Mr Pankaj Sharma1, Dr. Ankita Salunke1, Ms. Nishi Pandya1, Dr. Parth Pandya2, Prof. Pragna Parikh1
1Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, India, 2Department of Biomedical and Life Science, School of Science, Navrachana University, Vadodara, India
Biography:
Mr. Pankaj Sharma is an enthusiastic and committed entomologist who is now working towards his Ph.D. in the study of insecticide resistance mechanisms in stored pests. He is conducting his research at the Department of Zoology, The Maharaja Sayajirao University of Baroda in Vadodara, Gujarat. His profound expertise in insect biodiversity, insecticide resistance, and toxicological studies has made an important contribution. Mr. Pankaj Sharma has authored and co-authored over five scientific publications and two book chapters and has presented his research at more than ten international conferences. His commitment to professional growth is evident through his active participation in numerous workshops and training programs. Additionally, Mr. Pankaj Sharma is an active member of several Indian and international entomological societies, demonstrating his dedication to staying connected with the global scientific community.
Abstract:
The Callosobruchus chinensis (pulse beetles) from the family Bruchidae are serious store grain pest of pulses. 60% loss in weight of the pulses is due to them and hence not appropriate for human consumption and nor for planting. Insecticides are widely used to control pulse beetles, but these insecticides have adverse effects on the environment as well as non-target organisms and the pulse beetle becomes resistant to the insecticide due to continues exposure. Deltamethrin concentrations ranging from 1 to 1,000 ppm were sprayed on filter paper in the glass Petri dishes. After the filter paper dried, 20 adult insects were placed to determine the contact toxicity of deltamethrin. After obtaining Lethal concentration (LC50), the insects for were exposed to low dose (1/20th) and high dose (1/5th) of the LC50 and Control for seven days in successive generations and the transgenerational effects of the deltamethrin on C. chinensis was studied. Both concentrations significantly reduced the life table parameters like egg count, egg laying and hatching percentage, incubation period, total development duration, total emergence and adult longevity in the first generation but an increasing trend in all the parameters was observed in successive generations. Further, repellent effect was also recorded which shows that it was time and generation dependent. The repellency was maximum in the 1st generation, but it decreases with the exposure time and following generations. To confirm the findings currently we are performing the comparative transcriptome analysis of Callosobruchus chinensis (L.) between the control and the deltamethrin treated.
